The standard functionality of the alert mechanism does not provide for this. You can however, try using a pre-processing script that will throw an exception if no file is found at the specified time. Use Availability Time planing mechanism to active your sender adapter at the desired point of time.
You can also refer the following links for more help on this.
Create a ccBPM process. When the scenario starts, have the Sender channel as one of the steps and put a timeout for the correlation. Since no messages will come, correlation won't be met and it can then trigger an alert by going to the exception branch.
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