Hello
this is very generic error, more details must be known to solve the issue, if you are having this problem with your RFC communication channel, goto communicatin channel monitoring in RWB and there you would have complete details of why this error is occuring, do post back with those details to have clear understanding of the problem.
Moreover try creating an ABAP connection to R/3 system to which connection is being made and check if you are able to access the system altogether or not, for this follow the following step:
login to your PI server from SAP GUI with user PISUPER
got t-code SM59
Create destination with type: 3
provide ip address of your R/3 system in target host and instance number in respective field
save and execute "Connection test"
this will let you know if you are altogether able to access the R/3 system for PI server.
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